Sensitive and Simple Methods for Determination ofl-Lysine withl-Lysineα-Oxidase
- 1 August 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 43 (8), 1749-1752
- https://doi.org/10.1080/00021369.1979.10863679
Abstract
L-Lysine could be determined satisfactorily with a new fungal enzyme, l-Iysine α-oxidase (EC 1.4.3). The method consists of the oxidative deamination of l-Iysine with l-lysine α-oxidase and the spectrophotometric determination of one of the reaction products: α-keto-ε-aminocaproate, its intramolecular dehydrated form, Δ1-piperideine-2-carboxylate or hydrogen peroxide. The method on the basis of the color reaction of hydrogen peroxide formed from l-lysine with 4-aminoantipyrine and phenol in the presence of peroxidase was most sensitive and simple. The method could be used for the direct assay of l-lysine levels in serums from several animals without pretreatments.This publication has 4 references indexed in Scilit:
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