Direct determination of cis-dichlorodiammineplatinum(II) in urine by derivative spectroscopy.

Abstract

A derivative spectrophotometric method for the determination of the antitumor agent cis-dichlorodiammineplatinum(II) in human urine has been developed. The method is based on the complexation of cis-dichlorodiammineplatinum(II) with N,N''-bis(3-mercapto-2-quinoxalinyl)-1,3-propanediamine. Optimum conditions for quantification of cis-dichlorodiammineplatinum(II) were established. The complex formed was stable in urine, and the recovery of platinum from urine was 92-95%. Interference arising from urine can be avoided by measurement of the second-order derivative spectrum. The relative standard deviations of the present method were 3.9% and 1.9% for the determination of 0.2 and 2.0 ppm of platinum in urine, respectively.