Canonical Eukaryotic Initiation Factors Determine Initiation of Translation by Internal Ribosomal Entry
- 1 December 1996
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 16 (12), 6859-6869
- https://doi.org/10.1128/mcb.16.12.6859
Abstract
Translation of picornavirus RNA is initiated after ribosomal binding to an internal ribosomal entry site (IRES) within the 59 untranslated region. We have reconstituted IRES-mediated initiation on encephalomyocarditis virus RNA from purified components and used primer extension analysis to confirm the fidelity of 48S preinitiation complex formation. Eukaryotic initiation factor 2 (eIF2), eIF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-binding protein stimulated this process. We show that eIF4F binds to the IRES in a novel cap-independent manner and suggest that cap- and IRES-dependent initiation mechanisms utilize different modes of interaction with this factor to promote ribosomal attachment to mRNA.Keywords
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