Deoxypentose Nucleic Acids and Nucleoproteins of Malignant Tissues. III. Electrophoretic Studies of the Deoxypentose Nucleic Acids of Lymphoma #1 and of Calf Thymus

Abstract

An investigation of the electrophoretic properties of the deoxypentose nucleates of calf thymus and of the strain A mouse lymphoma No. 1 is reported. Both nucleates exhibited a single electrophoretic boundary at all pH values from about 2 to 13 and in all the buffer systems used. The variation of electrophoretic mobility with pH was determined over this range in a series of buffers containing only monovalent ions; the mobility=pH curves of the two nucleates were practically identical under these conditions. Below pH 11.6 the mobility-pH curves closely paralleled the forward titration curves. Above pH 11.6 no rise of mobility was observed in the region of pH where titration of enolic groups occurs. Electrophoretic studies in buffers containing bivalent anions indicate that discrepancies between electrophoretic and titration behavior may arise from specific effects of the bivalent anions, possibly as a result of their binding by the nucleate.