Alterations in protein kinase activity following exposure of cultured human lymphocytes to modulated microwave fields
- 1 January 1984
- journal article
- research article
- Published by Wiley in Bioelectromagnetics
- Vol. 5 (3), 341-351
- https://doi.org/10.1002/bem.2250050307
Abstract
Cultures of human tonsil lymphocytes were exposed in a Crawford cell to a 450-MHz field (peak envelope intensity 1.0 mW/cm2), sinusoidally amplitude modulated (depth 80%) at frequencies between 3 and 100 Hz for periods up to 60 min. The Crawford cell was housed in a temperature-controlled chamber (35°C) and control cultures were placed in the same chamber. Activity of cAMP-dependent protein kinase relative to controls remained unaltered by fields modulated at 16 or 60 Hz with exposures of 15, 30, and 60 min. By contrast, total non-cAMP-dependent kinase activity fell to less than 50% of unexposed control levels after 15 and 30 min exposures, but, despite continuing field exposure, returned to control or preexposure levels by 45 and 60 min. A smaller reduction (20–25%) also occurred with 60-Hz modulation and was also restricted to exposure durations of 15 and 30 min. CW 450-MHz fields were without effect. Reduced enzyme activity occurred with 16-, 40-, and 60-Hz modulation frequencies, but not with 3-, 6-, 80-, or 100-Hz modulation. The specific identity of this kinase is unknown. This rapid but transient reduction in lymphocyte protein kinase activity restricted to modulation frequencies between 16 and 60 Hz and to less than 30 min exposure is consistent with „windowing”︁ with respect to modulation frequency and exposure duration.Keywords
This publication has 27 references indexed in Scilit:
- Effects of microwave exposure on the hamster immune system. I. Natural killer cell activityBioelectromagnetics, 1983
- Effects of microwave exposure on the hamster immune system. II. Peritoneal macrophage functionBioelectromagnetics, 1983
- Effects of microwaves and hyperthermia on capping of antigen‐antibody complexes on the surface of normal mouse B lymphocytesBioelectromagnetics, 1983
- Calcium binding proteins and cellular regulationLife Sciences, 1982
- Immunologic and hematopoietic alterations by 2,450‐MHz electromagnetic radiationBioelectromagnetics, 1980
- Induction of calcium‐ion efflux from brain tissue by radio‐frequency radiation: Effects of modulation frequency and field strengthRadio Science, 1979
- EFFECTS OF MODULATED VHF FIELDS ON THE CENTRAL NERVOUS SYSTEM*Annals of the New York Academy of Sciences, 1975
- MICROWAVE EFFECTS ON THE BLOOD‐FORMING SYSTEM WITH PARTICULAR REFERENCE TO THE LYMPHOCYTEAnnals of the New York Academy of Sciences, 1975
- Lymphoblastoid Transformation of Lymphocytes in vitro after Microwave IrradiationNature, 1967
- Effect of microwave radiation on the hemopoietic system of the ratToxicology and Applied Pharmacology, 1964