Mechanism of Particulate Carrier Reactions: V. ADSORPTION OF HUMAN GAMMA GLOBULIN TO 0{middle dot}2 MICRON DIAMETER LATEX PARTICLES AND THEIR AGGLUTINATION BY RHEUMATOID FACTOR

Abstract

Human gamma globulin (HGG) adsorption to 0"2 [mu] diameter latex particles (LP) is quite rapid with 98% of the total taken up within 5 minutes and the remainder in about 1 hour. Adsorption appears to be in the form of a double molecular layer with the outer layer easily desorbed by washing and with the inner layer more strongly held and not readily desorbed by washing. The amount of nondesorbable protein increases when the HGG preparation is heated before coating. HGG-coated washed 0"2 [mu] LP carry at least 2[center dot]4 X 103 protein molecules adsorbed to their surface, but only 7 or 8 rheumatoid factor molecules fixed per particle are sufficient for complete agglutination.