P-fimbriae of pyelonephritogenic Escherichia coli: Identification and chemical characterization of receptors
- 1 January 1983
- journal article
- Published by Springer Nature in Infection
- Vol. 11 (1), 61-67
- https://doi.org/10.1007/bf01651362
Abstract
PyelonephritogenicEscherichia coli isolates were found to be equal in their ability to adhere to uroepithelial cells and to cause agglutination specific for human erythrocytes. This adhesive capacity was not affected byD-mannose and was found to be mediated by a new class ofE. coli fimbriae named P-fimbriae. Using human erythrocytes of different blood groups, we found the receptor molecules for P-fimbriae to be associated with the P blood group antigens, i. e. glycosphingolipids corresponding to the P blood group antigens P, P1 and Pk. By agglutination studies of erythrocytes with various set-ups of these antigens as well as by inhibition studies using synthetic saccharide derivatives and by coating non-agglutinable erythrocytes with a synthetic glycolipid, the minimal receptor structure was identified as the α-D-Galp-(1–4)-β-D-Galp moiety of the carbohydrate portion of these glycosphingolipids. Similar experiments on isolated uroepithelial cells proved that these glycosphingolipids also constitute the receptors in the urinary tract. PyelonephritogeneEscherichia coli-Isolate besitzen gleichermaßen die Fähigkeit, an uroepithelialen Zellen zu haften und menschliche Erythrozyten spezifisch zu agglutinieren. Die Haftfähigkeit wird durch D-Mannose nicht beeinflußt; sie wird durch eine neuentdeckte Klasse vonE. coli-Fimbrien, sogenannten P-Fimbrien, vermittelt. Durch Testung von menschlichen Erythrozyten verschiedener Blutgruppen zeigte sich, daß die Rezeptormoleküle für die P-Fimbrien mit den Antigenen der Blutgruppe P assoziiert sind, Glykosphingolipiden, die den Antigenen P, P1 und Pk der Blutgruppe P entsprechen. Agglutinationstests mit Erythrozyten, die diese Antigene in unterschiedlicher Konstellation besitzen, Hemmtests mit synthetischen Saccharidderivaten sowie Beladen nicht agglutinierbarer Erythrozyten mit einem synthetischen Glykolipid führten zur Identifizierung der minimalen Rezeptorstruktur als α-D-Galp-(1–4)-β-D-Galp-Verbindung des Kohlenhydratanteils dieser Glykosphingolipide. Ähnliche Experimente an isolierten uroepithelialen Zellen bewiesen, daß diese Glykosphingolipide auch die Rezeptoren des Harntrakts darstellen.Keywords
This publication has 19 references indexed in Scilit:
- OCCURRENCE OF P-FIMBRIATED ESCHERICHIA COLI IN URINARY TRACT INFECTIONSThe Lancet, 1981
- STRUCTURE OF CARBOHYDRATE PART OF RECEPTOR ON HUMAN UROEPITHELIAL CELLS FOR PYELONEPHRITOGENIC ESCHERICHIA COLIThe Lancet, 1981
- Chemical identification of a glycosphingolipid receptor forEscherichia coliattaching to human urinary tract epithelial cells and agglutinating human erythrocytesFEMS Microbiology Letters, 1980
- THE PkANTIGEN AS RECEPTOR FOR THE HAEMAGGLUTININ OF PYELONEPHRITICESCHERICHIA COLIFEMS Microbiology Letters, 1980
- BINDING OF PURIFIED ESCHERICHIA COLI PILI TO HUMAN URINARY TRACT EPITHELIAL CELLSFEMS Microbiology Letters, 1980
- Adhesion ofEscherichia colito human periurethral cells correlated to mannose-resistant agglutination of human erythrocytesFEMS Microbiology Letters, 1979
- BACTERIAL ADHERENCE TO PERIURETHRAL EPITHELIAL CELLS IN GIRLS PRONE TO URINARY-TRACT INFECTIONSThe Lancet, 1978
- Adhesion to normal human uroepithelial cells of Escherichia coli from children with various forms of urinary tract infectionThe Journal of Pediatrics, 1978
- Immunochemical study of the human blood group P1, P, and Pk glycosphingolipid antigensBiochemistry, 1975
- Fimbriæ and adhesive properties in dysentery bacilliThe Journal of Pathology and Bacteriology, 1957