Serologic Analysis of the Antigenic Specificities of Simian Virus 40-Transformed Cells and Their Relationship to Tumor-Associated Transplantation Antigen2

Abstract

In tests with the isotopic antiglobulin technique, the antisera produced by immunization of (BALB/c × C57BL)F₁ mice with syngeneic simian virus 40 (SV40) tumor (SVT2) reacted against at least 2 different specificities. Some sera only reacted with the tumor-associated cell-surface antigen (TASA) of SV40-transformed cells. Other sera also reacted with a common antigen found in non-SV40 tumor cells but not in normal spleen cells. Only TASA was found to be correlated with the tumor-associated transplantation antigen. Although all sera reacted with various SV40-transformed cells, they had different patterns of reactivity. These results indicate that the SV40 TASA may be composed either of different molecules or of single molecules with several antigenic determinants.