Human cytomegalovirus: glycoproteins associated with virions and dense bodies
- 1 August 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 19 (2), 594-609
- https://doi.org/10.1128/jvi.19.2.594-609.1976
Abstract
The glycoproteins associated with the membranes of cytomegalovirions and dense bodies were characterized by their relative mobility, percentage of glucosamine incorporation and MW. Eight glcopolypeptides were repeatedly detectable. Three glycopolypeptides of higher MW with low levels of glucosamine incorporation were occasionally detectable. These latter glycopolypeptides may be precursors or aggregates of the glycopolypeptides with lower MW. The glycoproteins associated with the membranes were on the surface, as determined by iodination with 125I of virions and dense bodies partially purified in gradients of D-sorbitol. Velocity centrifugation in linear gradients of D-sorbitol was used to obtain concentrated and partially purified preparations of infectious cytomegalovirus. Viral infectivity and the membranes of cytomegalovirions and dense bodies were stable in gradients of sorbitol, but cellular contaminants were not completel removed. Addition centrifugation in CsCl separated both cellular contaminants and viral nucleocapsids from virions and dense bodies. Many dense bodies, which are considered to be aberrant forms of cytomegalovirus, had the same size, sedimentation properties and density as virions. Consequently, they were not separable from virions by various centrifugation techniques. EM demonstrated that purified virions and dense bodies were qualitatively free of extraneous material and that each dense body was bounded by a membrane, as evidence by its double-track appearance. Antisera to a preparation of purified virions and dense bodies, or to their glycoproteins, contained antibodies that neutralized viral infectivity and reacted with antigens in cells infected with cytomegalovirus. These same antisera did not contain antibodies that reacted with uninfected cells. The glycoproteins associated with the membranes of ctomegalovirions and dense bodies are considered to be specified by the cytomegalovirus genome. [WI-38 human fetal lung fibroblasts and human foreskin fibroblasts were used.].This publication has 44 references indexed in Scilit:
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