Active and passive re‐expression of Fcγ receptors on human lymphocytes
- 1 May 1980
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 10 (5), 352-358
- https://doi.org/10.1002/eji.1830100507
Abstract
Modulation of receptors for IgG (FcγR) on human lymphocytes was induced by the interaction with erythrocyte‐IgG antibody (EAG) complexes followed by incubation at 37°C. The re‐expression of FcγR could be achieved by two independent processes, (a) Active synthesis, susceptible to inhibition by puromycin or cycloheximide was shown to peak 4 to 6 h after removal of EAG complexes; it required addition of at least 2% fetal calf serum. (b) Insertion of soluble FcγR into the membrane of modulated lymphocytes was shown to occur within 20 min of contact between cells and FcγR‐containing supernatants; it was not altered by protein synthesis inhibitors. FcγR‐like material, spontaneously released by unstimulated peripheral blood lymphocytes or by polymorphonuclear cells, was taken up by modulated lymphocytes. This soluble material was fully absorbed on polymerized human IgG; it was non‐ dialyzable, thermolabile (56 °C, 30 min) and partially destroyed by freezing and thawing; it was recovered as two broad peaks from chromatography on polyacrylamide gel; it was shown to bind to both T and non‐T FcγR‐bearing lymphocytes capable of forming EAG rosettes before modulation; and it could be inserted into allogeneic lymphocytes. These results demonstrate that the FcγR structure bears two active sites, one binding to the Fcγ and the other to the surface of EAG rosette‐forming cells. Rapid release of soluble FcγR from the cells and their possible insertion into the membrane may have important implications with respect to the biological functions associated with these receptors.This publication has 35 references indexed in Scilit:
- MODULATION OF LYMPHOCYTE RECEPTORS FOR IgG INHIBITS RESPONDING CELLS IN THE MIXED LYMPHOCYTE REACTIONTransplantation, 1979
- Insoluble immune complexes suppress mitogen-induced proliferation of human T lymphocytes bearing Fcγ receptorsCellular Immunology, 1979
- Isolation and characterization of a human mononuclear cell Fc receptorImmunochemistry, 1978
- Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexesNature, 1978
- Presence of “la‐like” antigens on human T lymphocytes bearing receptors for IgGEuropean Journal of Immunology, 1977
- Distribution of Cells Binding Erythrocyte‐Antibody (EA) Complexes in Human Lymphoid PopulationsScandinavian Journal of Immunology, 1976
- Suppression of in vitro antibody synthesis by immunoglobulin-binding factor.The Journal of Experimental Medicine, 1975
- A human lymphoid cell line secreting immunoglobulin G and retaining immunoglobulin M in the plasma membraneCell, 1975
- Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants.The Journal of Experimental Medicine, 1975
- Immunoglobulin-binding factor present on and produced by thymus-processed lymphocytes (T cells)Cellular Immunology, 1974