Determining the inhibition constants in the HCH-1 model of cellulose hydrolysis

Abstract

The products of cellulose hydrolysis, glucose and cellobiose, caused noncompetitive inhibition patterns to be exhibited when Thermomonospora sp. YX cellulase hydrolyzed dyed cellulose. The glucose binding constant, β₁, was 0.00683 ± 0.00031 L/g and the cellobiose binding constant, β₂, was 0.095 ± 0.0058 L/g. Thus, cellobiose was about 14 times more inhibitory than glucose.