Enzyme Electrophoresis as an Alternative Method for Separating the Sympatric Leishmania Vectors Phlebotomus papatasi and Phlebotomus langeroni (Diptera: Psychodidae)
- 1 September 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 27 (5), 773-776
- https://doi.org/10.1093/jmedent/27.5.773
Abstract
Trials were conducted to determine the accuracy of separating the sympatric sand fly species Phlebotomus papatasi (Scopoli) and P. langeroni Nitzulescu by means of cellulose acetate enzyme electrophoresis. Malic enzyme, phosphoglucomutase, 6-phosphogluconate dehydrogenase, and fumarate hydratase were each evaluated in laboratory-reared and field-collected populations of the two species. Each of the four enzyme-based identifications was highly sensitive (>97%) and specific (>93%). Identifications based upon fumarate hydratase were in perfect agreement with morphological identifications, and evidence was obtained which indicates that this enzyme may be the most stable of the four enzymes tested. The application of enzyme-based vector identification is discussed in relation to classical and novel survey procedures for Leishmania promastigote detection in sand flies.Keywords
This publication has 7 references indexed in Scilit:
- Electrophoretic Comparison of the Leishmania Vectors Phlebotomus papatasi and P. langeroni (Diptera: Psychodidae)Journal of Medical Entomology, 1990
- Biochemical identification of Phlebotomus (larroussius) pedifer and Phlebotomous (larroussius) elegonensisBiochemical Systematics and Ecology, 1988
- Detection of Leishmania within Sand Flies by Kinetoplast DNA HybridizationThe American Journal of Tropical Medicine and Hygiene, 1988
- The biology ofPhlebotomus langeroni(Diptera : Psychodidae) under laboratory conditionsAnnales de Parasitologie Humaine et Comparée, 1987
- Sand Flies (Diptera: Psychodidae) Associated with Visceral Leishmaniasis in El Agamy, Alexandria Governorate, Egypt1Journal of Medical Entomology, 1986
- Sand Flies (Diptera: Psychodidae) Associated with Visceral Leishmaniasis in El Agamy, Alexandria Governorate, Egypt1Journal of Medical Entomology, 1986
- Monoclonal antibodies that distinguish between New World species of LeishmaniaNature, 1981