An Inexpensive Microtome Attachment for Cutting 50–Micron Nonfrozen Sections for Electron Microscopic Histochemistry

Abstract

The attachment is for an American Optical Co., Model 888 freezing microtome. It consists of right-angle bracket made from % inch metal rod, one end of which M clamped in the freezing stage holder; the other supports a tissue holder whose base lies at a right angle to the usual position. The fixed and blotted tissue specimen is enclosed (without infiltration) in parah, on a piece of filter paper which is attached to the base of the tissue holder, and sectioned across its long axis by 50 μ increments. After sectioning, the filter paper is removed from the base, the paraffin matrix opened, and the sections transferred to the appropriate processing fluid