Analysis of betamethasone, dexamethasone and related compounds by liquid chromatography/electrospray mass spectrometry
- 23 February 2004
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 18 (6), 678-684
- https://doi.org/10.1002/rcm.1386
Abstract
A reversed-phase high-performance liquid chromatography/electrospray ionisation mass spectrometry (HPLC/ESI-MS) method has been developed to conclusively differentiate the epimers betamethasone and dexamethasone and various esterification products (betamethasone and dexamethasone 21-acetate, betamethasone and dexamethasone 21-phosphate, betamethasone 17-valerate, betamethasone 21-valerate and betamethasone 17,21-dipropionate) in counterfeit drugs. Good separation with baseline resolution of all epimers or isomers was obtained on a Zorbax Eclipse XDB or Luna C8 column, using a step gradient with mobile phases of 0.05 M ammonium acetate and acetonitrile. Betamethasones can also be distinguished by the relative abundance of their m/z 279 ion in the positive electrospray tandem mass spectra. The LC/MS or LC/MS/MS method developed was successfully applied to the analysis of drug product samples, i.e. creams and tablets. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
This publication has 16 references indexed in Scilit:
- Validation of a screening method for corticosteroids in doping analysis by liquid chromatography/tandem mass spectrometryRapid Communications in Mass Spectrometry, 2003
- Sensitive liquid chromatographic/tandem mass spectrometric method for the determination of beclomethasone dipropionate and its metabolites in equine plasma and urineJournal of Mass Spectrometry, 2003
- A high‐performance liquid chromatography/tandem mass spectrometric screening method for eight synthetic corticosteroids in bovine feces and the simultaneous differentiation between dexamethasone and betamethasoneRapid Communications in Mass Spectrometry, 2002
- Method for confirmation of synthetic corticosteroids in doping urine samples by liquid chromatography–electrospray ionisation mass spectrometryJournal of Chromatography A, 2001
- Screening for anabolic steroids and related compounds in illegal cocktails by liquid chromatography/time‐of‐flight mass spectrometry and liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry with accurate mass measurementRapid Communications in Mass Spectrometry, 2001
- Simultaneous determination of betamethasone and dexamethasone residues in bovine liver by liquid chromatography/tandem mass spectrometryRapid Communications in Mass Spectrometry, 2001
- Simultaneous determination of dexamethasone and betamethasone in pharmaceuticals by reversed-phase HPLCChromatographia, 1994
- Optimization of the high-performance liquid chromatographic separation of a mixture of natural and synthetic corticosteroidsJournal of Chromatography B: Biomedical Sciences and Applications, 1993
- Determination of betamethasone and dexamethasone by derivitization and liquid chromatographyAnalytica Chimica Acta, 1992
- Stereochemical analysis of betamethasone and dexamethasone by derivatization and high-performance liquid chromatographyJournal of Chromatography A, 1992