Intravesical Prostaglandin: Release and Effect of Bladder Instillation on some Micturition Parameters

Abstract

Bladder washout effluent fluid was examined for prostaglandin[PG]-like activity following acidic lipid extraction and bioassay using the rat-stomach strips technique. A low basal level of PG-like material was detected in washouts collected when the bladder was empty in 4 of 7 dogs. Increased levels were detected following micturition in 5 of these dogs. A significantly high level of PG-like material was recovered after pelvic nerve stimulation. The PG-like material was tentatively identified as PGE, based on the bioassay with the addition of specific antibody. Intravesically administered PGE2 or PGF2.alpha. significantly reduced, in a dose-dependent response, the threshold micturition volume. These findings suggest that PG may play a role in the micturition process.