A Two-Component Multidrug Efflux Pump, EbrAB, in Bacillus subtilis
- 15 April 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (8), 2307-2310
- https://doi.org/10.1128/jb.182.8.2307-2310.2000
Abstract
Genes (ebrAB) responsible for ethidium resistance were cloned from chromosomal DNA of Bacillus subtilis ATCC 9372. The recombinant plasmid produced elevated resistance against ethidium bromide, acriflavine, pyronine Y, and safranin O not only inEscherichia coli but also in B. subtilis. It also caused an elevated energy-dependent efflux of ethidium in E. coli. EbrA and EbrB showed high sequence similarity with members of the small multidrug resistance (SMR) family of multidrug efflux pumps. Neither ebrA nor ebrB was sufficient for resistance, but introduction of the two genes carried on different plasmids conferred drug resistance. Thus, both EbrA and EbrB appear to be necessary for activity of the multidrug efflux pump. In known members of the SMR family, only one gene produces drug efflux. Thus, EbrAB is a novel SMR family multidrug efflux pump with two components.Keywords
This publication has 36 references indexed in Scilit:
- Negative Dominance Studies Demonstrate the Oligomeric Structure of EmrE, a Multidrug Antiporter from Escherichia coliJournal of Biological Chemistry, 1996
- Genes acrA and acrB encode a stress‐induced efflux system of Escherichia coliMolecular Microbiology, 1995
- EmrE, an Escherichia coli 12-kDa Multidrug Transporter, Exchanges Toxic Cations and H+ and Is Soluble in Organic SolventsJournal of Biological Chemistry, 1995
- Multidrug resistance pumps in bacteria: variations on a themeTrends in Biochemical Sciences, 1994
- High-level resistance to ethidium bromide and antiseptics in Staphylococcus aureusFEMS Microbiology Letters, 1992
- High-level resistance to ethidium bromide and antiseptics inStaphylococcus aureusFEMS Microbiology Letters, 1992
- Cupric ions induce both an efflux of potassium and low molecular mass metabolites inPseudomonas syringaeFEMS Microbiology Letters, 1990
- Nucleotide sequence and comparative analysis of the frd operon encoding the fumarate reductase of Proteus vulgarisEuropean Journal of Biochemistry, 1987
- Analysis of membrane and surface protein sequences with the hydrophobic moment plotJournal of Molecular Biology, 1984
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983