Isolation of Antibodies to Gelatin from Antigen-Antibody Complex by Proteolysis

Abstract

Antibodies were isolated by digestion of the gelatin-antigelatin complex with collagenase and removal of the gelatin fragments by dialysis. The pure antibody could be precipitated anew by the antigen. Antibodies to tyrosylgelatin were isolated in a similar way, but in this case peptides containing the active antigenic sites remained bound to the antibody.