Utility of Cyclodextrin in Mobile Phase for High-Performance Liquid Chromatographic Separation of C21Steroids

Abstract
The chromatographic behavior of C21 steroids, especially corticoids, and its fluorescent derivatives with anthroyl cyanide was examined by the reversed-phase high-performance liquid chromatography. The separation of the compounds was much improved by the addition of the suitable cyclodextrin to the mobile phase.