The Separation of Conjugated and Unconjugated Bilirubin in Bile by High-Performance Liquid Chromatography
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 2 (1), 37-43
- https://doi.org/10.1080/01483917908060043
Abstract
A fast and simple method was developed for the separation of unconjugated bilirubin and its mono- and di-glucuronide conjugates from bile by high-performance liquid chromatography (HPLC). Unconjugated bilirubin was separated on a reversed-phase column using acetonitrile-water (70:30 vol/vol) as the mobile phase, while the conjugates were separated on a .mu.-Bondapak-carbohydrate column employing acetonitrile-water (90:10 vol/vol) as the eluent. The application of this method was demonstrated by the analysis of the bile pigments in rat bile.This publication has 6 references indexed in Scilit:
- Recent Advances in the Separation and Analysis of Diazo‐Positive Bile PigmentsMethods of Biochemical Analysis, 1974
- Heterogeneity of bile pigment conjugates as revealed by chromatography of their anthranilate azopigmentsBiochemical Journal, 1970
- THE IDENTIFICATION OF “DIRECT-REACTING” BILIRUBIN AS BILIRUBIN GLUCURONIDEJournal of Biological Chemistry, 1957
- The excretion of bilirubin as a diglucuronide giving the direct van den Bergh reactionBiochemical Journal, 1957
- Properties and Composition of the Bile Pigment giving a Direct Diazo ReactionNature, 1956