The 3′terminal oligonucleotide of E.coli 16S ribosomal RNA: the sequence in both wild-type and RNase III cells is complementary to the polypurine tracts common to mRNA initiator regions
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6), 787-798
- https://doi.org/10.1093/nar/2.6.787
Abstract
Application of Sanger techniques to the analysis of the 3′ terminal oligonucleotide from E. coli32P-labelled 16S rRNA yields the sequence AUCACCUCCUUAOH. This sequence is identical in RNA isolated from two wild-type strains (MRE600 and E. coli B, SY106) and from a mutant strain (AB301/105) defective in RNase III. Data presented here explains the previous derivation of an incorrect sequence (AUCCUCACUUCAOH) by others. The functional significance of complementarity between the 3′ terminus of 16S rRNA and poly-purine tracts commonly found in mRNA initiator regions is discussed.Keywords
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