DERIVATIZATION OF CONTROLLED PORE GLASS-BEADS FOR SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS
- 1 September 1988
- journal article
- research article
- Vol. 6 (8), 768-775
Abstract
An improved and simplified procedure for the attachment of nucleosides onto long chain alkylamine controlled pore glass bead (LCAA-CPG) is presented. This procedure uses 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (DEC) to couple nucleoside 3''-succinates directly to the LCAA-CPG. The preparation of nucleoside 3''-succinate anhydrides, p-nitrophenyl, or pentachlorophenyl esters and the use of highly toxic dicyclohexylcarbodiimide (DCC) is not longer required. Procedures involving acidic activation of the LCAA-CPG before derivatization and a pre-synthesis capping are also described, which prevent the formation of oligonucleotides linked by 3''-phosphates to the LCAA-CPG. Evidence is presented indicating that his type of linkage is responsible for the apparently greater than 100% coupling yields observed for the first coupling cycle.This publication has 8 references indexed in Scilit:
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