Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis
- 1 November 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 159 (2), 317-322
- https://doi.org/10.1042/bj1590317
Abstract
Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage λ DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage λ chromosome at which the 5'-terminal sequence is pPy-C-G-N. AvaII endonuclease cuts phage λ DNA more extensively, yielding fragments with the 5'-terminal sequence G-T-C-N or G-A-C-N. Neither enzyme generates cohesive ends.This publication has 23 references indexed in Scilit:
- Restriction Endonucleases in the Analysis and Restructuring of DNA MoleculesAnnual Review of Biochemistry, 1975
- An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange celluloseAnalytical Biochemistry, 1974
- The behaviour of oligodeoxynucleotides on thin-layer chromatography on polyethyleneimine-cellulose and ion-exchange paper electrophoresis. Applications in fractionating and sequencing terminally labelled oligodeoxynucleotidesBiochemical Journal, 1974
- The Primary Structure of DNAProgress in Nucleic Acid Research and Molecular Biology, 1974
- Recognition Sequence of a Restriction EnzymeNature New Biology, 1973
- Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresisBiochemistry, 1973
- Nucleotide sequence analysis with polynucleotide kinase and nucleotide ‘mapping’ methods. 5′-Terminal sequence of deoxyribonucleic acid from bacteriophages λ and 424Biochemical Journal, 1973
- Cleavage of DNA by R 1 Restriction Endonuclease Generates Cohesive EndsProceedings of the National Academy of Sciences, 1972
- The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdgJournal of Molecular Biology, 1960
- Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica LemmPlant Physiology, 1955