Transplantation Antigens (H-2) on Virally and Chemically Transformed BALB/3T3 Fibroblasts in Culture2

Abstract

Quantitative absorptions of monospecific and oligospecific H-2^d antisera were used to test differences in concentration of histocompatibility antigens on nontransformed BALB/3T3 (clone A31) mouse fibroblasts; on A31 fibroblasts transformed by Kirsten murine sarcoma virus (MSV), simian virus 40 (SV40), and 3-methylcholanthrene; and on spontaneously transformed A31 fibroblasts. Cells transformed spontaneously and by MSV showed increased H-2 concentration, whereas those transformed chemically and by SV40 had decreased H-2 antigen specificities. The findings correlated with the lower in vivo immunogenicity of cells transformed spontaneously and by MSV and with the higher immunogenicity of cells transformed chemically and by SV40. An inverse relationship between tumor-associated and histocompatibility antigens was suggested.