Studies in the secretion of the pancreas and salivary glands

Abstract

Observations on the living pancreas cell (mouse and frog) showed that the secretory granules first arose in the basal region of the cell in close relation to the mitochondria. Emigration to the Golgi zone then occurred where subsequent de-velopment took place. Immature granules stained with vital dyes (Janus green and neutral red), the dye being secreted into the perigranular vacuole. Overstaining of the cell with these vital dyes led to gross enlargement of these vacuoles, giving rise to the Krinom bodies of other authors. In mature granules the staining capacity was lost. A comparison of fixed and vitally stained preparations of the salivary gland structures of the rat showed that a similar process occurred in the cells of these glands. A description of the types of salivary gland cell found in this animal is included.