X‐ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1‐exohydrolase IIa ofCichorium intybus
- 14 December 2004
- journal article
- Published by Wiley in The Plant Journal
- Vol. 41 (3), 400-411
- https://doi.org/10.1111/j.1365-313x.2004.02304.x
Abstract
Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32).Keywords
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