Synthesis of a substrate of protein kinase C and its corresponding phosphopeptide
- 1 February 1989
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 33 (2), 115-123
- https://doi.org/10.1111/j.1399-3011.1989.tb00196.x
Abstract
The syntheses of a protein kinase C (PKC) peptide substrate, H-Lys-Arg-Thr-Leu-Arg-OH, and a phosphopeptide analog of the synthetic substrate, H-Lys-Arg-Thr(P)-Leu-Arg-OH, are reported. PKC phosphorylates the peptide with an apparent KM of 0.30 ± 0.04 mM and an apparent Vmax equal to one-tenth that of histone III-S. The synthesis of the phosphopeptide features a recently developed convenient phosphorylation procedure for serine and threonine using N,N-diethylamino-dibenzylphosphoramidite. A complete characterization of the PKC substrate and its corresponding phosphopeptide by C-H COSY 2D n.m.r. is included.Keywords
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