A Staining Method for Nematodes: Determination of Nematode Resistant Stages and Direct Counts from Soil
- 1 April 1979
- journal article
- research article
- Published by JSTOR in Transactions of the American Microscopical Society
- Vol. 98 (2), 213-218
- https://doi.org/10.2307/3226021
Abstract
Because of the selectively permeable nature of nematode [Mesodiplogaster lheritieri, Acrobeloides sp. and Mononchus spp.] cuticles, staining of nematodes has been difficult. The effect of pronase and collagenase on cuticle permeability to 5 stains was discussed. Collagenase increased the rate of staining in both laboratory and field populations of dead nematodes. Live nematodes did not absorb stains even when treated with the enzymes. Nematodes must be either heat-killed or fixed in a preservative. Biebrich scarlet-eosin B, a red dye, was absorbed most rapidly, and nematodes stained were visible in soils. This allowed direct count of nematode populations in soil samples.This publication has 4 references indexed in Scilit:
- EFFECTS OF HYDROLYTIC ENZYMES ON PLANT-PARASITIC NEMATODES1977
- [47] PronasePublished by Elsevier ,1970
- Dynamics of the permeation of nematodes by water, nematocides and other substancesExperimental Parasitology, 1968
- Factors Influencing the Extraction of Nematodes From SoilNematologica, 1966