Calcium Influx in a Single Rat Basophilic Leukemia Cell as Revealed with a Digital Imaging Fluorescence Microscope1
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 102 (1), 1-4
- https://doi.org/10.1093/oxfordjournals.jbchem.a122020
Abstract
Using a digital imaging fluorescence microscope, we have detected a rapid transient increase in the free cytosolic calcium concentration in a single rat basophilic leukemia cell (RBL-2H3) after antigen stimulation. Calcium ions were transported very rapidly (within 1 s) after a lag time (about 10 s at 37°C) from the external environment into the cytoplasm. On the basis of the present experimental results we conclude that the gradual changes in the overall fluorescence intensity observed for a cell suspension are due to the distribution of different lag times shown by different cells as to the calcium influx through membrane calcium channels.This publication has 9 references indexed in Scilit:
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