Über Peroxydase. I. Mitteilung. Allgemeines und Untersuchungsmethodik.

Abstract

The methods for quantitative estimation of peroxidase action (oxidation by H2O2 of pyrogallol to purpurogallin, of guaiacol to tetraguaiacol, and of o-cresol to a yellow brown substance of unknown constitution) are discussed critically. When tested with ferment preparations of unlike origin and unequal purity, the 3 methods give like results, except that guaiacol oxidation lags behind the others with the increasing purity of the preparation. Guaiacol oxidation is initiated by substances associated with the enzyme, among which iron is important.