Precursors of the pyrimidine moiety of thiamine
- 1 January 1968
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 106 (1), 271-277
- https://doi.org/10.1042/bj1060271
Abstract
1. A method was devised for obtaining the pyrimidine moiety of thiamine in a pure form after its excretion into the medium by de-repressed washed-cell suspensions of mutants of Salmonella typhimurium LT2. 2. By using amino acid-requiring mutants, this excretion of pyrimidine moiety was shown to be dependent on the presence of both methionine and glycine. 3. In the presence of either [Me−14C]methionine or [G−14C]methionine, methionine-requiring mutants did not incorporate radioactivity into the pyrimidine moiety. 4. In contrast, both [1−14C]glycine and [2−14C]glycine were incorporated into the pyrimidine moiety excreted by glycine-requiring mutants, and this occurred with little or no dilution of specific radioactivity. 5. The possible requirement for methionine as a cofactor and the significance of the incorporation of both carbon atoms of glycine are discussed.This publication has 23 references indexed in Scilit:
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