Oxazine Dyes. I. Celestine Blue B with Iron as a Nuclear Stain
- 1 January 1956
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 31 (4), 141-150
- https://doi.org/10.3109/10520295609113794
Abstract
It is suggested that celestine blue B can stain as a colloidal dispersion, the nuclear specificity of which is controlled by the pH. The staining solution is prepared by adding 0.5 ml of concentrated H2SO4 to 1 gm of celestine blue B and dissolving the resultant granular mass in 100 ml of 2.5% ferric alum containing 14 ml of glycerol. Sections of amphibian, avian, and mammalian tissue placed for 1 min in this solution and then rinsed in water show as sharp nuclear staining as that usually produced by hematoxylin. A wide variety of fixatives is permissible. Overstaining is not possible within reasonable limits of exposure and no differentiation nor bluing is required. Both the staining solution and stained slides are stable.Keywords
This publication has 4 references indexed in Scilit:
- The microtomist's formulary and guidePublished by Biodiversity Heritage Library ,1954
- Handbook of basic microtechniquePublished by Biodiversity Heritage Library ,1952
- Celestin blue as a nuclear stainThe Journal of Pathology and Bacteriology, 1935
- Metallic Lakes of the oxazines (Gallamin Blue, Gallocyanin and Coelestin Blue) as Nuclear Stain Substitutes for HematoxylinStain Technology, 1928