The sequence of the mu transmembrane segment determines the tissue specificity of the transport of immunoglobulin M to the cell surface.
Open Access
- 1 March 1990
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 171 (3), 947-952
- https://doi.org/10.1084/jem.171.3.947
Abstract
Membrane IgM is expressed on the surface of B lymphocytes. It is not transported to the surface of transfected plasmacytoma or COS cells. Here, we show that mutation of four hydrophilic amino acids in the microm transmembrane is sufficient to overcome the intracellular retention of membrane IgM in non-B cells. This suggests that the B cell-specific IgM-associated proteins that have been postulated to assist the transport of membrane IgM to the cell surface (3) act either by forming a hydrophobic sheath that surrounds the microm transmembrane segment or by displacing an interaction with this segment that would otherwise cause retention. Experiments with a CD8/mu hybrid H chain indicate that the proteins that assist the transport of membrane IgM to the B cell surface at most need the mu CH4 and transmembrane/cytoplasmic portion for interaction.This publication has 12 references indexed in Scilit:
- B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties.The EMBO Journal, 1988
- A novel 34-kd protein co-isolated with the IgM molecule in surface IgM-expressing cells.The EMBO Journal, 1988
- Transfected plasmacytoma cells do not transport the membrane form of IgM to the cell surface.The Journal of Experimental Medicine, 1988
- The intron requirement for immunoglobulin gene expression is dependent upon the promoterNucleic Acids Research, 1988
- Regulation of membrane IgM expression in secretory B cells: translational and post-translational events.The EMBO Journal, 1987
- Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequenceCell, 1985
- The isolation and sequence of the gene encoding T8: A molecule defining functional classes of T lymphocytesCell, 1985
- The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens.The EMBO Journal, 1983
- Establishment and Characterization of BALB/c Lymphoma Lines with B Cell PropertiesThe Journal of Immunology, 1979