Phospholipase A2 (PLA2) activity in rabbit chondrocytes
- 1 June 1989
- journal article
- Published by Springer Nature in Inflammation Research
- Vol. 27 (3-4), 385-387
- https://doi.org/10.1007/bf01972829
Abstract
The effects of recombinant interleukin-1β (rIL-1β), recombinant tumor necrosis factor (rTNFα) and two growth factors, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on PLA2 activity and prostaglandin E2 (PGE2) release were investigated using rabbit chondrocytes. Cellular PLA2 activity increased 2–10×above controls in the presence of 8×10−12 M (5 U) rIL-1β or 5×10−9 M rTNFα after 20 hr incubation. PLA2 activity remained constant with 1–50 ng/ml of either growth factor. PGE2 release significantly increased (p2 activity and PGE2 release are not coordinately regulated in rabbit chondrocytes.Keywords
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