Some Quantitative Aspects of Scanning Densitometry in High-Performance Liquid Chromatography
- 19 December 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (16), 2875-2926
- https://doi.org/10.1080/01483918508076609
Abstract
The optimization of instrumental parameters that influence resolution and signal-to-noise ratios in the recording of thinlayer chromatograms by scanning densitometry are discussed. A standardized method of determining sensitivity and detectability is proposed and used to evaluate the performance of a Shimadzu CS-910 scanning densitometer. Fluorescence enhancement reagents are discussed as a method of improving sample detectability in the fluorescence mode. The influence of the sorbent medium on both the position and intensity of the fluorescence emission signal is discussed.Keywords
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