Purification of tonin by affinity chromatography.
- 1 January 1981
- journal article
- research article
- Published by Wolters Kluwer Health in Hypertension
- Vol. 3 (1), 81-86
- https://doi.org/10.1161/01.hyp.3.1.81
Abstract
Tonin has been purified from rat submaxillary glands. The purification procedure included affinity chromatography on Sepharose 4B coupled to antitonin followed by DEAE chromatography and gel filtration on Sephadex G-100. Homogeneity of the purified enzyme was confirmed by Sephadex G-100 gel filtration, disc electrophoresis, and isoelectric focusing on polyacrylamide gel, immunodiffusion, and immunoelectrophoresis. The tonin was purified 11.5-fold, with 35% recovery. The purified tonin has full enzymatic or immunological activity.This publication has 13 references indexed in Scilit:
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