Distribution of Mutarotase in Subfractions of Rat Intestinal Mucosa and Rat Kidney.

Abstract

1. A method for assaying the enzyme mutarotase in rat kidney and rat intestine was developed. 2. Kidney contains considerably more enzyme than intestine and the activity appears to be concentrated in the tubules. 3. Intestinal mucosa was fractionated into brush border and supernatant fractions by the method of Crane. Brush border membrane contained most of the mucosal invertase but little of the mutarotase. Ninety per cent of the mutarotase remained in a supernatant fraction after removal of brush border. 4. The possible implications of these findings in connection with mechanism of sugar transport are discussed.