Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by anin vivo diffusion chamber method
- 1 December 1984
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 36 (1), 83-86
- https://doi.org/10.1007/bf02405298
Abstract
Rabbit bone marrow has been separated into core, intermediate, and endosteal cell populations. When plated outin vitro, each of the fractions gave rise to colonies of fibroblastic cells. The colony-forming efficiency increased from the core population by a factor of 4 to a maximum of 3.4 × 10−6 in the endosteal fraction. The osteogenic potential of each fraction was determined following their implantation in diffusion chambers into host rabbits. Each of the indices of osteogenesis (alkaline phosphatase activity, Ca and P accumulation) were significantly lower in the core population than in the two populations closer to the bone surface. Our results are consistent with the hypothesis that the osteogenic precursor cells of marrow belong to the fibroblast colony-forming cell fraction, and indicate that these cells, although found throughout the marrow, are concentrated close to the bone surface.Keywords
This publication has 16 references indexed in Scilit:
- Assessment of anin vivo diffusion chamber method as a quantitative assay for osteogenesisCalcified Tissue International, 1984
- Osteogenesis and leukopoiesis within diffusion‐chamber implants of isolated bone marrow subpopulationsJournal of Anatomy, 1980
- The origin of bone cells in the postnatal organismArthritis & Rheumatism, 1980
- Stromal Mechanisms of Bone Marrow: Cloning in Vitro and Retransplantation in VivoPublished by Springer Nature ,1980
- Endosteal Marrow: A Rich Source of Hematopoietic Stem CellsScience, 1978
- Precursor Cells of MechanocytesInternational Review of Cytology, 1976
- Measurement of DNA in cultured human cellsAnalytical Biochemistry, 1974
- Transplantation of Marrow to Extramedullary SitesScience, 1968
- Microdetermination of PhosphorusAnalytical Chemistry, 1956
- On a Test of Whether one of Two Random Variables is Stochastically Larger than the OtherThe Annals of Mathematical Statistics, 1947