A rapid method for the determination of salicylate binding by the use of ultrafilters

Abstract

An ultrafiltration method that combines speed, simplicity, accuracy and precision was employed to measure plasma binding of salicylate. Human plasma, containing 1·0 and 0·1mm sodium salicylate, was ultrafiltered through membranes using 45 p.s.i. and slow rotational stirring. Salicylate was bound at these concentrations 13·1 and 4·4% respectively. This method, using membranes that quantitatively separate small molecules from plasma proteins, yields results comparable to those obtained by equilibrium dialysis.