A method for the comparative quantitative extraction of 24-hr, collections of human urine was devised. No comb growth stimulating hormone was found in urine from boys under 10 yrs. of age. The urine of adolescent boys and of pregnant and normal women, yielded quantities of hormone of the same order as the urine from mature men. The comb growth response from pregnancy urine could not be duplicated by the action of theelin. The comb growth stimulating hormone and the theelin in women''s urine were separated from each other by the ether-10% NaOH treatment.