The Enzymic Degradation of an Alkali-soluble Glucan from the Cell Walls of Saccharomyces cerevisiae
- 1 February 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 98 (2), 315-327
- https://doi.org/10.1099/00221287-98-2-315
Abstract
An alkali-soluble glucan from the cell walls of S. cerevisiae NCYCI 109 has been hydrolyzed with a purified endo-(1 .fwdarw. 3)-.beta.-D-glucanase and an endo-(1 .fwdarw. 6)-.beta.-D-glucanase from Bacillus circulans WL-12. The products of enzyme action include various oligosaccharide and polysaccharide fractions which were separated by gel filtration and characterized, giving new information on the fine structure of the glucan. The isolated cell walls were also subjected to enzymic hydrolysis. Part of the cell-wall mannan may be held in place by a glucan component.Keywords
This publication has 3 references indexed in Scilit:
- Isolation and Composition of an Alkali-soluble Glucan from the Cell Walls of Saccharomyces cerevisiaeJournal of General Microbiology, 1976
- Enzymatic Hydrolysis of Yeast Cell Walls I. Isolation of Wall-Decomposing Organisms and Separation and Purification of Lytic EnzymesJournal of Bacteriology, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951