THE NATURE OF THE GRAM COMPOUND AND ITS BEARING ON THE MECHANISM OF STAINING
- 1 October 1930
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 20 (4), 287-295
- https://doi.org/10.1128/jb.20.4.287-295.1930
Abstract
A study of absorption spectra and boiling point elevations shows that the compound precipitated out when the dye, crystal violet, and iodine combine in aqueous solution, redissociates into free dye and free iodine when the precipitate is dissolved in acetone or alcohol. This indicates that any theory of the mechanism of the Gram reaction based on the presence of a large staining molecule formed between dye and mordant and stable in presence of decolorizer can not be valid, and furnishes confirmation of the authors'' chemical theory of its mechanism.This publication has 7 references indexed in Scilit:
- THE CELL WALL AND THE GRAM REACTIONJournal of Bacteriology, 1929
- The Variation in Staining Character of Bacteria as Related to the Reserve Food Material Within the OrganismStain Technology, 1929
- The Effect of the Chemical Nature of a Decolorizer on Its Functioning. II. the Apparent Isoelectric PointStain Technology, 1928
- THE CHEMICAL MECHANISM OF BACTERIAL BEHAVIOR II. A NEW THEORY OF THE GRAM REACTIONJournal of Bacteriology, 1924
- THE CHEMICAL MECHANISM OF BACTERIAL BEHAVIOR I. BEHAVIOR TOWARD DYES—FACTORS CONTROLLING THE GRAM REACTIONJournal of Bacteriology, 1924
- A new method for differential staining of bacteriaThe Journal of Infectious Diseases, 1922