Selective staining of fungal hyphae in parasitic and symbiotic plant-fungus associations
- 1 March 1988
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 88 (2), 197-199
- https://doi.org/10.1007/bf00493305
Abstract
A cytochemical method for light microscopical studies is described which allows the specific detection of fungal hyphae in plant-fungus associations: e.g. lichens, mycorrhiza, or fungal infections of plant tissue. The specimens were fixed and embedded in epoxy resin by a standard protocol for electron microscopy. Semithin sections were successively incubated with fluorescein isothiocyanate labelled wheat germ agglutinin (FITC-WGA) and calcofluor white (CW). FITC-WGA stained exclusively the fungal cell walls while CW stained both the fungal and the plant cell walls. Therefore, FITC-WGA is an excellent marker for the fungal hyphae.Keywords
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