Computer Simulation Study of Hexokinase II from Ehrlich Ascites Cells
- 1 February 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 51 (2), 437-447
- https://doi.org/10.1111/j.1432-1033.1975.tb03943.x
Abstract
A study of the mechanism of hexokinase II from ascites cells the effects of its binding to mitochondrial membranes has been carried out by computer simulation. This is based on experimental data of Kosow and Rose and of Gumaa and McLean, and the theoretical methods of cleveland. For the soluble enzyme the mechanism is random with ternary produce-inhibition complexes; when bound to mitochondria, the mechanism becomes ordered-on, random-off, as the binding of ATP to the free enzymes becomes negligibly slow. The requirements of experimental data for mechanistic studies are discussed.Keywords
This publication has 39 references indexed in Scilit:
- Mechanisms of enzyme control as probed by equilibrium exchange rates: Patterns of modifier effects with a two substrate, two product systemJournal of Theoretical Biology, 1973
- Origin of the delayed feedback control of glucose utilization in ascites tumor cellsBiochemical and Biophysical Research Communications, 1972
- Simulation of enzyme systems using a matrix representationComputers and Biomedical Research, 1970
- Transient isolation of the hexokinase reaction from the glycolytic sequence on initiation of glycolysis in ascites tumor cellsBiochemical and Biophysical Research Communications, 1970
- Sequential control of hexokinase in ascites cellsBiochemical and Biophysical Research Communications, 1969
- The simulation and analysis by digital computer of biochemical systems in terms of kinetic models IV. Automatic derivation of enzymic rate lawsJournal of Theoretical Biology, 1968
- Simulation of the detailed regulation of glycolysis in a heart supernatant preparationComputers and Biomedical Research, 1968
- The practicality of the use of the steady-state assumption and the inflection point method in enzyme kineticsJournal of Theoretical Biology, 1967
- Studies of the Enzyme Hexokinase. I. Steady State Kinetics at pH 8Journal of the American Chemical Society, 1962
- A direct study of intracellular glycolysis in ehrlich's ascites tumorBiochimica et Biophysica Acta, 1959