F plasmid ccd mechanism in Escherichia coli
Open Access
- 1 April 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 166 (1), 100-104
- https://doi.org/10.1128/jb.166.1.100-104.1986
Abstract
The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same culture. Elongated cells and anucleate cells caused by the ccd mechanism were clearly detected by flow cytometry in cultures of bacterial strains harboring Ccd+ Sop- mini-F plasmids defective in partitioning. This indicates that the defect in correct partitioning of plasmid DNA molecules into daughter cells also induces the ccd mechanism to operate.This publication has 17 references indexed in Scilit:
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