The metabolic requirements for the accumulation of (3H)-noradrenaline by rabbit atria and vas deferens have been studied. It was found that inhibition of either glycolysis or oxidation via the Krebs cycle alone had little effect on amine accumulation but inhibition of both processes greatly reduced accumulation of (3H)-noradrenaline by both tissues. Glycolysis was inhibited by iodoacetate and 2-deoxy-D-glucose. D-Glucose, D-mannose, pyruvate, lactate, oxaloacetate and β-hydroxy-butyrate could be utilised for the energy requirements of amine transport but D-galactose, D-fructose, 2-deoxy-D-glucose, 3-O-methyl-glucose and succinate were not.