Coordination of editing and splicing of glutamate receptor pre-mRNA
Open Access
- 1 March 2003
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 9 (3), 309-318
- https://doi.org/10.1261/rna.2750803
Abstract
Adenosine deaminase that acts on RNA, ADAR, catalyzes the conversion of adenosine into inosine within double-stranded RNA. This type of editing has mainly been found in genes involved in neurotransmission. Site-specific A to I modifications often require intronic sequences to create the double-stranded structure necessary for editing. A system was developed to investigate if editing and splicing of pre-mRNA are coordinated. We have focused on a selectively edited site (R/G) in the glutamate receptor subunit B pre-mRNA. This editing site is situated in close proximity to a 5′ splice site. To ensure efficient splicing, the editing site, together with its natural 5′ splice site, was fused to a 3′ splice site of the major late transcript from adenovirus. In vitro, on a premade transcript, ADAR2 editing and splicing were found to interfere with each other. The stable stem-loop required for ADAR2 editing had a negative effect on in vitro splicing, possibly by sequestering the 5′ splice site. Further, RNA helicase A was shown to overcome the splicing inhibition caused by ADAR2. In vivo, allowing cotranscriptional processing, the same construct was found to efficiently edit and splice without interference, suggesting that the two RNA processing events are coordinated.Keywords
This publication has 35 references indexed in Scilit:
- An extensive network of coupling among gene expression machinesNature, 2002
- Dual Roles of RNA Helicase A in CREB-Dependent TranscriptionMolecular and Cellular Biology, 2001
- Active Disruption of an RNA-Protein Interaction by a DExH/D RNA HelicaseScience, 2001
- Changing genetic information through RNA editingBioEssays, 2000
- The mlenapts RNA Helicase Mutation in Drosophila Results in a Splicing Catastrophe of the para Na + Channel Transcript in a Region of RNA EditingNeuron, 2000
- Coupling RNA polymerase II transcription with pre-mRNA processingCurrent Opinion in Cell Biology, 1999
- Regulation of serotonin-2C receptor G-protein coupling by RNA editingNature, 1997
- Structural Requirements for RNA Editing in Glutamate Receptor Pre-mRNAs by Recombinant Double-stranded RNA Adenosine DeaminaseJournal of Biological Chemistry, 1996
- A mammalian RNA editing enzymeNature, 1996
- Divalent ion permeability of AMPA receptor channels is dominated by the edited form of a single subunitNeuron, 1992