An Application of the Prussian Blue Technique to a Light Microscope Study of Water Movement in Transpiring Leaves of Cotton (Gossypium hirsutumL.)

Abstract

Prussian blue deposition in transpiring leaves of Gossypium hirsutum L. indicated that water moved rapidly from the tracheary elements by a path along the bundle sheath parenchyma to the bundle sheath extension parenchyma and then laterally through the epidermis to the sub-stomatal cavities or to the tnchomes. A slower pathway of water movement to the substomatal cavities occurred through the mesophyll. The path of Prussian blue deposition indicated that waterleft the leaf through the stomata and to a lesser extent through the trichomes. In the outer periclinal wall of epidermalcells Prussian blue was deposited below but not within the cuticle.