Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.
- 1 February 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (2), 415-419
- https://doi.org/10.1073/pnas.73.2.415
Abstract
The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of reassociation kinetics as measured with hydroxyapatite and with single strand specific nuclease. The free parameter that describes the extent of inhibition of nucleation with single strand tails in these equations has been evaluated by least squares methods and agrees with the experimentally measured value.This publication has 4 references indexed in Scilit:
- Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.Proceedings of the National Academy of Sciences, 1975
- Sequence organization in Xenopus DNA studied by the electron microscopeJournal of Molecular Biology, 1975
- Excluded volume effects on the rate of renaturation of DNABiopolymers, 1971
- Kinetics of renaturation of DNAJournal of Molecular Biology, 1968