The γ subunit in the Escherichia coli ATP synthase complex (ECF1F0) extends through the stalk and contacts the c subunits of the F0 part
- 17 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (2), 235-238
- https://doi.org/10.1016/0014-5793(95)00658-v
Abstract
A mutant, in which a cysteine has been site‐directed into the polar loop region of the c subunit at residue 44, has been studied. Cross‐linking of the c subunit to both the γ and ϵ subunits was observed with cupric 1,10‐phenanthrolinate treatment. The linkage between the c and γ subunits was localized to that part of the γ subunit between residues 202–286, based on peptide analysis. Reference to the high resolution structure of F1 [Abrahams et al. (1994) Nature 370, 621–628] appears to limit this contact site to the region including residues 202–230. This segment contains 4 tyrosines and 1 tryptophan as possible reactive residues for cross‐linking with the c subunit cysteine.Keywords
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