IMMUNOFLUORESCENCE IN THE STUDY OF VIRUSES IN TISSUE CULTURE: I. DEVELOPMENT OF AN IMMUNOFLUORESCENT FOCUS ASSAY FOR HERPES SIMPLEX VIRUS
- 1 November 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 13 (11), 1489-1497
- https://doi.org/10.1139/m67-197
Abstract
A rapid and sensitive assay method has been developed for herpes simplex virus in kidney cells of Cercopithecus aethiops. Using immunofluorescence, foci of infection produced by this virus can be scored within 20–22 hours after inoculation. It has been demonstrated that this procedure gives reproducible results and exhibits a direct relationship between virus concentration and the number of fluorescent foci. The technique is slightly more sensitive than the conventional plaque assay method using the same host system.This publication has 20 references indexed in Scilit:
- The rapid detection, titration, and differentiation of variola and vaccinia viruses by a fluorescent antibody-coverslip cell monolayer systemVirology, 1965
- A Quantitative Cell Culture Assay for Yaba Tumour VirusNature, 1964
- Plaque Assay for Measurement of Cells Infected with Zoster VirusScience, 1963
- Studies on the assay and multiplication of avian myeloblastosis virusVirology, 1963
- A Sensitive and Precise Plaque Assay for Herpes VirusNature, 1962
- EFFECT OF MULTIPLICITY OF INFECTION ON NEWCASTLE DISEASE VIRUS-HELA CELL INTERACTIONThe Journal of Experimental Medicine, 1961
- The Adsorption, Penetration and Eclipse of Herpes Simplex Virus in Chorio-Allantoic Membrane EctodermJournal of General Microbiology, 1960
- 'Shortened Latency' as a Result of Multiple Infection by Vesicular Stomatitis Virus in Chick Cell CultureJournal of General Microbiology, 1958
- The formation of microscopic plaques by herpes simplex virus in HeLa cellsVirology, 1958
- The interactions of Newcastle disease virus (NDV) with chick embryo tissue culture cells: Attachment and growthVirology, 1956